During the process of
DNA replication, errors occasionally occur in the polymerization of the second strand. These errors, called
mutations, can have an impact on the phenotype of an organism, especially if they occur within the protein coding sequence of a gene. Error rates are usually very low1 error in every 10100 million basesdue to the "proofreading" ability of
DNA polymerases.
[56][57] (Without proofreading error rates are a thousandfold higher; because many viruses rely on DNA and RNA polymerases that lack proofreading ability, they experience higher mutation rates.) Processes that increase the rate of changes in DNA are called
mutagenic: mutagenic chemicals promote errors in DNA replication, often by interfering with the structure of base-pairing, while
UV radiation induces mutations by causing damage to the DNA structure.
[58] Chemical damage to DNA occurs naturally as well, and cells use
DNA repair mechanisms to repair mismatches and breaks in DNAnevertheless, the repair sometimes fails to return the DNA to its original sequence.
In organisms that use
chromosomal crossover to exchange DNA and recombine genes, errors in alignment during
meiosis can also cause mutations.
[59] Errors in crossover are especially likely when similar sequences cause partner chromosomes to adopt a mistaken alignment; this makes some regions in genomes more prone to mutating in this way. These errors create large structural changes in DNA sequence
duplications,
inversions or
deletions of entire regions, or the accidental exchanging of whole parts between different chromosomes (called
translocation).
And more.