A later study funded by the Smithsonian Institution, the results of which were released in 2019, concluded from the mitochondrial DNA of his grandniece, known injuries, and physical characteristics, that the skeleton was likely Pulaski's. The skeleton has a number of typically female features, which has led to the hypothesis that Pulaski may have been female or intersex. A documentary based on the Smithsonian study suggests that Pulaski's hypothesized intersex condition could have been caused by congenital adrenal hyperplasia, where a fetus with female chromosomes is exposed to a high level of testosterone in utero and develops partially male genitals. This analysis was based on the skeleton's female pelvis, facial structure and jaw angle, in combination with the fact that Pulaski identified as and lived as male.
However, there is no conclusive argument or evidence that Pulaski was intersex. The question remains unsettled due to the limited understanding of how an intersex condition might be revealed in the analysis of a skeleton. There is no way to prove that Pulaski was born intersex without a DNA